Post-doctoral fellow, UCSD
Skaggs School of Pharmacy and Pharmaceutical Sciences
The focus of my research is neglected tropical diseases, in particular development of novel therapeutics for schistosomiasis. My interest in neglected tropical diseases began during PhD studies in the laboratory of Dr. Colette Dissous at the Center of Immunity and Infection in Lille, France. This training gave me a strong background in the S. mansoni parasite model and regulation of the cell cycle. While studying cell cycle regulation by Polo-like kinases I gained expertise in protein kinase structure and developed skills in in vivo inhibition assays and various protein expression systems, including Xenopus oocyte model, mammalian cells and bacteria. As a postdoctoral scholar in Dr. James McKerrow's laboratory I expanded my interest to a number of other proteins, including proteases, kinases and enzymes in the mevalonate pathway. These targets are not only essential for parasite survival but also are involved in host-pathogen interactions. To identify and validate potential vaccine and drug candidates to prevent or treat schistosomiasis, I employ a variety of different techniques, including recombinant protein expression in bacteria and yeast, kinetic assays, crystallization, RNA interference and phenotypic screening.
Beckmann S, Quack T, Burmeister C, Buro C, Hahnel S, Long T, Dissous C, Grevelding CG. Sex in schistosomes - signaling mechanisms in the female gonads. H. Mehlhorn (ed.), Progress in Parasitology, Parasitology Research Monographs 2, Springer-Verlag Berlin Heidelberg 2011. DOI 10.1007/978-3-642-21396-0_10
Phenotypic, chemical and functional characterization of cyclic nucleotide phosphodiesterase 4 (PDE4) as a potential anthelmintic drug target. PLoS Negl Trop Dis. 2017 Jul 13;11(7):e0005680. doi: 10.1371/journal.pntd.0005680. eCollection 2017 Jul. Long T, Rojo-Arreola L, Shi D, El-Sakkary N, Jarnagin K, Rock F, Meewan M, Rascón AA Jr, Lin L, Cunningham KA, Lemieux GA, Podust L, Abagyan R, Ashrafi K, McKerrow JH, Caffrey CR.
Algorithmic Mapping and Characterization of the Drug-Induced Phenotypic-Response Space of Parasites Causing Schistosomiasis. IEEE/ACM Trans Comput Biol Bioinform. 2018 Mar-Apr;15(2):469-481. doi: 10.1109/TCBB.2016.2550444. Epub 2016 Apr 7. Singh R, Beasley R, Long T, Caffrey CR.
Structure-Bioactivity Relationship for Benzimidazole Thiophene Inhibitors of Polo-Like Kinase 1 (PLK1), a Potential Drug Target in Schistosoma mansoni. PLoS Negl Trop Dis. 2016 Jan;10(1):e0004356. doi: 10.1371/journal.pntd.0004356. Long T, Neitz RJ, Beasley R, Kalyanaraman C, Suzuki BM, Jacobson MP, Dissous C, McKerrow JH, Drewry DH, Zuercher WJ, Singh R, Caffrey CR.
Serum albumin and α-1 acid glycoprotein impede the killing of Schistosoma mansoni by the tyrosine kinase inhibitor Imatinib. Int J Parasitol Drugs Drug Resist. 2014 Dec;4(3):287-95. doi: 10.1016/j.ijpddr.2014.07.005. Beckmann S, Long T, Scheld C, Geyer R, Caffrey CR, Grevelding CG.
Chemical and genetic validation of the statin drug target to treat the helminth disease, schistosomiasis. PLoS One. 2014;9(1):e87594. doi: 10.1371/journal.pone.0087594. Rojo-Arreola L, Long T, Asarnow D, Suzuki BM, Singh R, Caffrey CR.
SmSak, the second Polo-like kinase of the helminth parasite Schistosoma mansoni: conserved and unexpected roles in meiosis. PLoS One. 2012;7(6):e40045. doi: 10.1371/journal.pone.0040045. Epub 2012 Jun 29. Long T, Vanderstraete M, Cailliau K, Morel M, Lescuyer A, Gouignard N, Grevelding CG, Browaeys E, Dissous C.
Schistosoma mansoni Polo-like kinases and their function in control of mitosis and parasite reproduction. An Acad Bras Cienc. 2011 Jun;83(2):627-35. Dissous C, Grevelding CG, Long T.
Schistosoma mansoni Polo-like kinase 1: A mitotic kinase with key functions in parasite reproduction. Int J Parasitol. 2010 Aug 1;40(9):1075-86. doi: 10.1016/j.ijpara.2010.03.002. Epub 2010 Mar 27. Long T, Cailliau K, Beckmann S, Browaeys E, Trolet J, Grevelding CG, Dissous C.
Schistosoma mansoni: signal transduction processes during the development of the reproductive organs. Parasitology. 2010 Mar;137(3):497-520. doi: 10.1017/S0031182010000053. Epub 2010 Feb 18. Review. Beckmann S, Quack T, Burmeister C, Buro C, Long T, Dissous C, Grevelding CG.
[A new promising drug against schistosomiasis]. Med Sci (Paris). 2009 Jan;25(1):24-6. doi: 10.1051/medsci/200925124. French. No abstract available. Dissous C, Ahier A, Long T.